• Girija P.V.
• Dr K K Vijayan
• Dr. N K Renuka

Abstract: The healthy state of our body is maintained by equilibrium between the oxidative stress and the antioxidant systems of our body. Oxidative stress occurs due to the imbalance between the productions of reactive oxygen species (ROS) and the availability of the antioxidant or radical scavengers. The excess ROS produced can structurally alter proteins/gene. This in turn will lead to the onset and progression of inflammatory diseases. The inflammation triggered by oxidative stress is the cause of many chronic diseases such as heart diseases, AIDS, Rheumatoid arthritis, neurodegenerative diseases, and cancer and in the aging process. Gulguluthikthakam Kashayam and Prasarinyadi Kashayam are well known Ayurvedic formulations usually prescribed for the treatment of many chronic ailments such as arthritis, diabetes, autoimmune diseases. The present study was to evaluate anti-oxidant activity of methanol extract of Gulguluthikthakam Kashayam and Prasarinyadi Kashayam. The antioxidant studies were conducted using DPPH assay, hydroxyl radical scavenging assay and Ferric iron reducing antioxidant power assay. Phytochemical screening reveal the presence of alkaloids, flavanoids ,phenolics compounds and steroids as major constituents and amino acids ,sugars, glycosides and proteins as minor constituents. The FRAP assay reveals the dose dependent increase in absorbance. The IC50 value calculated in the DPPH assay and Hydroxyl radical scavenging assay. The IC50 value found to be lowest in the methanol extract of Gulguluthikthakam Kashayam residue (MGP), 317.333µgmL-1 in DPPH assay and 525.196µgmL-1 in Hydroxyl radical scavenging assay, which is lower than that of positive control used. Low IC50 value indicates high antioxidant property. Highest anti oxidant property shown by MGP compared to all other extracts.

Gulguluthikthakam Kashayam, Prasarinyadi Kashayam, anti-oxidant, DPPH, FRAP, Free radicals, hydroxyl radical scavenging ,Mannitol, Ayurvedic formulation

"Radical scavenging activity of Gulguluthikthakam Kashayam and Prasarinyadi Kashayam", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 8, page no.2199-2215, August-2020, Available :http://www.jetir.org/papers/JETIR2008291.pdf

"Radical scavenging activity of Gulguluthikthakam Kashayam and Prasarinyadi Kashayam", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 8, page no. pp2199-2215, August-2020, Available at : http://www.jetir.org/papers/JETIR2008291.pdf