• Mahesh A. Suryawanshi
• V.B. Mane
• Gaurav P Malshikare
• Ajinkya Mohite
• Bhavesh Birwadkar

Ursolic acid is a triterpene molecule present widely in the medicinal plants. The major sources include tulsi leaves (Ocimum sanctum) and nilgiri leaves (Eucalyptous alba). Ursolic acid is having many biological activities including anti-diabetic, muscle building, antimicrobial activity, anti-obesity etc. Ursolic acid is being used in ayurvedic and nutraceutical formulations. The current project involves the process development for extraction and purification of ursolic acid from tulsi leaves. The extraction process development includes optimization of different process parameters such as solid-solvent ratio, time, agitation speed, solvent used for extraction etc. The optimization would done to make the process more efficient in terms of selectivity, yield and productivity. The crude extract obtained after extraction contains many other compounds other than ursolic acid as extraction is not that selective for natural products. The presence of other compounds may increase or decrease the activity of ursolic acid. Hence the further purification is required to get pure ursolic acid.The purification process involves the use of different synthetic adsorbents and silica column chromatography. The optimization of chromatographic purification will be done with respect to the flow rate, feed volume, concentration of feed, fraction volume, column height, column volume, composition of mobile phase etc.Characterization of the purified product will be done using HPLC, LC-MS, FTIR and NMR.

Extraction, optimization, Chromatography, composition.

"EXTRACTION AND PURIFICATION OF URSOLIC ACID FROM TULSI LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 9, page no.592-597, September-2020, Available :http://www.jetir.org/papers/JETIR2009079.pdf

"EXTRACTION AND PURIFICATION OF URSOLIC ACID FROM TULSI LEAVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 9, page no. pp592-597, September-2020, Available at : http://www.jetir.org/papers/JETIR2009079.pdf