• Dr. Rajib Mukherjee

The objective of this work was to evaluate the “Effect of various process parameters on production and activity of α–Amylase by two Bacillus spp. namely B.amyloliquefaciens MTCC 1488 and B.licheniformis MTCC 1483 using household vegetable wastes as a substrate in solid state fermentation”. Maximum enzyme titer obtained was 3467 U/g with the optimized production parameters i.e. after 72 hours of fermentation at 370C with the initial pH 4.0, initial moisture of 85% when the substrate inoculated with B.amyloliquefaciens of 2-109 CFU/ml; whereas 4419 U/g in case of B.licheniformis with the optimized production parameters, the only difference lies in temperature at 500C with the initial pH 7.5, initial moisture of 70%. Supplementation with 1% soluble starch showed maximum enzyme production in both cases, more so by B.licheniformis (5647 U/g). Supplementation of different nitrogen sources showed no significant effect on enzyme production by both strains. Among metal salts, enzyme production in both cases increased only when substrate supplemented with 0.1% Cacl2. Optimum α–Amylase activity of B.amyloliquefaciens was observed at 600C, pH 7.0 and in presence of 0.15% Cacl2; whereas in B.licheniformis only difference lies in temperature i.e. 700C. However, both the strains of Bacillus retailed more than 90% enzyme activity upto 1000C compared to control 500C.

Alpha Amylase, Bacillus amyloliquefaciens, B.licheniformis, Solid state fermentation, Household vegetable wastes.

"Effect of various parameters on The Production and activity of α–amylase by two Bacillus spp. namely B.amyloliquefaciens MTCC 1488 and B.licheniformis MTCC 1483 using household argo-wastes as a substrate in solid state fermentation. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.1170-1176, November-2020, Available :http://www.jetir.org/papers/JETIR2011296.pdf

"Effect of various parameters on The Production and activity of α–amylase by two Bacillus spp. namely B.amyloliquefaciens MTCC 1488 and B.licheniformis MTCC 1483 using household argo-wastes as a substrate in solid state fermentation. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp1170-1176, November-2020, Available at : http://www.jetir.org/papers/JETIR2011296.pdf