• Amrita Masanta
• K.Gopinath Achary
• Shikha Singh

Evaluation of the clinical usefulness for detection of isolated serum from infected tuberculosis (TB) individuals was raised against mycobacterial antigenic protein molecular weight of 14 KDa, 16KDa, 110KDa and 190 KDa for possibility of robust diagnosis & discrimination between tuberculosis and other pulmonary diseases. Under optimum experiment conditions, mycobacterial antigenic proteins was identified by using indirect ELISA with high sensitivity detection limit of 0.1 mg at 1:5000 dilution. Statistical analysis report shows the correlation coefficient among various phases of tuberculosis infected urine samples assembled from Balasore, Odisha, India. Proteins ranges from 14KDa -190KDa were got separated and identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Western blotting results shows positive reaction against the mycobacterial antigenic proteins & also revealed immune-reactive bands with high antibody retort contrast to the proteins with molecular weight of 35KDa, 66KDa and 110KDa, along with 4 additional reactive bands molecular weight of 14KDa, 16KDa, 110KDa, and 190KDa. The outcomes suggest that the TB serum could be fortunately employed for the specific immunological detection against mycobacterial antigenic proteins at an early phase of tuberculosis infection.

Tuberculosis; mycobacterial antigenic protein; TB serum; SDS-PAGE; indirect ELISA; Western blotting

"Monitoring the advancement and evaluation of a novel diagnostic method for rapid detection of tuberculosis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 11, page no.126-135, November-2020, Available :http://www.jetir.org/papers/JETIR2011316.pdf

"Monitoring the advancement and evaluation of a novel diagnostic method for rapid detection of tuberculosis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 11, page no. pp126-135, November-2020, Available at : http://www.jetir.org/papers/JETIR2011316.pdf