UGC Approved Journal no 63975(19)

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Published in:

Volume 7 Issue 12
December-2020
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2012278


Registration ID:
304036

Page Number

582-590

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Title

Isolation and characterization of Botrytis antigen from Allium cepa L. and its role in rapid diagnosis of Neck rot Diseases.

Abstract

Early detection and perfect diagnosis of neck rot in onions authorized for early treatment which can increase production and storage. In the current status, polyclonal antibody (pAb) raised against the protein extract from Botrytis allii was fixed for the detection of neck rot using serological assays. The pathogenic proteins were identified by ELISA with high sensitivity (60 ng). Correlation efficient between infected onions from different agroclimatic stages with antibody titers were taken as the primary endpoint for standard protocol. The highest positive correlation (r ¼ 1.502) was observed in stage I infected samples of the south zone, whereas a low negative correlation (r ¼ _0.087) was found in stage III infected samples of the south zone among developed pAb. Linear positive correlations between antigenic proteins at all the stages of infection and the developed pAb titers. Hence, at an early stage, the developed pAbs should be used to detect the presence of the fungal pathogen. This point of study recommends the use of developed antibodies in rapid diagnosis of neck rot in onions.

Key Words

Neck rot, onions, Botrytis allii, Polyclonal antibody, ELISA, rapid diagnosis

Cite This Article

"Isolation and characterization of Botrytis antigen from Allium cepa L. and its role in rapid diagnosis of Neck rot Diseases.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 12, page no.582-590, December-2020, Available :http://www.jetir.org/papers/JETIR2012278.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Isolation and characterization of Botrytis antigen from Allium cepa L. and its role in rapid diagnosis of Neck rot Diseases.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 12, page no. pp582-590, December-2020, Available at : http://www.jetir.org/papers/JETIR2012278.pdf

Publication Details

Published Paper ID: JETIR2012278
Registration ID: 304036
Published In: Volume 7 | Issue 12 | Year December-2020
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.25351
Page No: 582-590
Country: Bhubaneswar, Odisha, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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