• NAIKAWADI VIJAY BANDU
• NAIKAWADI VIKAS BANDU

Lobelia Nicotineafolia (Campulanaceae ; Rann tambaku) is a rare high altitude wild tobacco species. This species is generally found in monsoon season. Ethno medicinally and local tribal people use this plant for treatment of cold and respiratory problem. This plant contains active metabolites lobeline, which comes under piperidine alkaloids. Natural population hamper due to low seed viability as well as germination rate. Biotechnological intervention through tissue culture play important role for conservation and rare endanger medicinal plant. MS media supplemented permutation and combination of cytokinine and auxins with 30 % sucrose plus application of cytokinin and auxin for induction of embryonic calli through leaves explant. This protocol would be beneficial for improvement of clonal propagation for sustainable source of lobeline.

Alkaloid, Ayurvedic system of medicines. Mass propagation, conservation, medicinal plant

"Embryonic Callus Induction of Lobelia nicotineafolia Roth: A Medicinal Wild Tobacco. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.7, Issue 12, page no.44-49, December-2020, Available :http://www.jetir.org/papers/JETIR2012410.pdf

"Embryonic Callus Induction of Lobelia nicotineafolia Roth: A Medicinal Wild Tobacco. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.7, Issue 12, page no. pp44-49, December-2020, Available at : http://www.jetir.org/papers/JETIR2012410.pdf