• Dr Mohit Mishra
• Mr Bhairav Prasad
• Dr Ashish Saraf
• Kamini Kashyap

Rhizobium is soil bacteria which are characterized morphologically as gram negative, small rods and motile. During nodule formation EPS is secreted by Rhizobium in addition to many plant growth promoting factor to access the root hair infection. A total 14 isolates were recovered and were designated as RZK1- RZK14. These isolates were morphologically and biochemically characterized and were gram negative, rod. Then EPS was isolated and estimated from these all 14 isolates and the highest producer viz. RZK7, RZK9 and RZK13 were subjected for further studies. The amount of EPS produced by these isolates was 500, 512 and 515µg/ml respectively. RZK13 grown in YEMM containing 2% mannitol have produced the highest amount of EPS (645μg/ml). PGPA tests were also performed for these three selected isolates. RZK7, RZK9 and RZK13 were found to be positive for siderophore test, phosphate solubilization, ammonia production, HCN production, Zn solubilization and cell wall degrading enzymes. Bioinoculation of isolate RZK13 was done in soyabean seeds. Plants grown from inoculated seeds shown fast growth in comparison to the control seeds. In 20 days, plants originated from the inoculated seeds have attained height of 18cm whereas control plants have attained height of 12cm only.

Key words: Exopolysaccharides, PGPA, Rhizobium

"Extracellular Polysaccharide production by Rhizobium Sp. with their plant growth pramoting activity isolated from cajanuscajan root nodules .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 3, page no.412-430, March-2021, Available :http://www.jetir.org/papers/JETIR2103060.pdf

"Extracellular Polysaccharide production by Rhizobium Sp. with their plant growth pramoting activity isolated from cajanuscajan root nodules .", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 3, page no. pp412-430, March-2021, Available at : http://www.jetir.org/papers/JETIR2103060.pdf