• Dr.K.Rajathi
• M.Muthubharathi
• K.B.Leneeygreen

Abstract: Biosynthesis of nanoparticles using algae extract is more advantageous over other biological processes such as bacteria and fungi, because it eliminates the process of cell culture and maintenance allows large-scale production of nanoparticles. Hence the present study is focused to determine the effect of Fucoidan Zinc oxide nanoparticle on cell cycle proliferation, cell cycle distribution and TRAIL induced apoptotic cell death in prostate cancer PC-3 cell line. The biosynthesized zinc oxide nanoparticle was characterized by UV-visible spectroscopy, Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectrum (FTIR) analysis. The cell cytotoxicity and apoptosis studies were conducted in this study. Formation of fucoidan zinc oxide nanoparticles was indicated by the color change from yellow to pale white. UV-Vis spectral analysis showed Surface Plasmon Resonance band at 342 nm. The SEM image showed that most of the zinc oxide nanoparticles are spherical in shape. EDX analysis confirmed the presence of elemental zinc signals of the nanoparticles. FTIR spectroscopy analysis was used to identify the possible biomolecules like alkenes, dicarboxylic amino acids, amides and nitro compounds in the wavelength range between 500 cm-1and 4000 cm-1. In vitro cytotoxicity of the Fucoidan Zinc oxide nanoparticles was screened against Prostate cancer PC-3 cell line cells at different concentrations (25-400µg/ml) and the viability of tumor cells was confirmed by using MTT assay. The IC 50 value of biosynthesized fucoidan zinc oxide nanoparticle was observed at 234.21µg/ml. From the results it was observed that biosynthesized silver nanoparticle inhibits the cellular growth of human prostate cancer PC-3 cells and induces apoptosis.

Fucoidan, Brown seaweeds, Zinc oxide nanoparticles, Apoptosis, Prostate cancer

"Effect of Fucoidan Mediated Zinc Oxide Nanoparticle on the TRAIL induced Apoptosis in Prostate Cancer Pc-3 Cell line", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 4, page no.391-398, April-2021, Available :http://www.jetir.org/papers/JETIR2104055.pdf

"Effect of Fucoidan Mediated Zinc Oxide Nanoparticle on the TRAIL induced Apoptosis in Prostate Cancer Pc-3 Cell line", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 4, page no. pp391-398, April-2021, Available at : http://www.jetir.org/papers/JETIR2104055.pdf