UGC Approved Journal no 63975(19)

ISSN: 2349-5162 | ESTD Year : 2014
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Published in:

Volume 8 Issue 5
May-2021
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2105064


Registration ID:
308683

Page Number

a546-a558

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Title

IN VITRO CYTOTOXICITY AND APOPTOSIS INDUCTION IN HUMAN OROPHARYNGEAL CARCINOMA KB CELLS BY PURIFIED ESTERASE ISOZYME EXTRACT OF URGINEA POLYPHYLLA HYACINTHACEAE.

Abstract

Plant extracts which are natural products having potent anticancer activity is good for treating cancer. The use of plants for the treatment and prevention of cancer is giving much attention in the medical field. Biologically active compounds extracted from medicinal plants are currently being evaluated as source of promising anticancer agents. They are important for the discovery of new drugs for cancer treatment. The aim of the present study was to investigate the anticancer activity of Purified Esterase Isozyme isolated from Urginea species on KB carcinoma cell of human mouth (Oral cancer) We report the anticancer effects of Urginea Polyphylla, a species of Urginea collected from Castle rock Poona exhibited anticancer activity against cell line KB epidermal carcinoma (Oral cancer). This study confirms the potency of its cytotoxic activity on KB cancer cell lines by MTT ([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The apoptosis-inducing activity by Annexin-V and Caspase 3 determination and its effect on mitochondrial membrane potential were measured by flow cytometry. Further DNA damage can be explained by TUNNEL assay.

Key Words

KB Cancer cell lines, Cytotoxicity, MTT, Annexin, Apoptosis, TUNEL, Caspase 3, flow cytometry

Cite This Article

"IN VITRO CYTOTOXICITY AND APOPTOSIS INDUCTION IN HUMAN OROPHARYNGEAL CARCINOMA KB CELLS BY PURIFIED ESTERASE ISOZYME EXTRACT OF URGINEA POLYPHYLLA HYACINTHACEAE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 5, page no.a546-a558, May-2021, Available :http://www.jetir.org/papers/JETIR2105064.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"IN VITRO CYTOTOXICITY AND APOPTOSIS INDUCTION IN HUMAN OROPHARYNGEAL CARCINOMA KB CELLS BY PURIFIED ESTERASE ISOZYME EXTRACT OF URGINEA POLYPHYLLA HYACINTHACEAE.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 5, page no. ppa546-a558, May-2021, Available at : http://www.jetir.org/papers/JETIR2105064.pdf

Publication Details

Published Paper ID: JETIR2105064
Registration ID: 308683
Published In: Volume 8 | Issue 5 | Year May-2021
DOI (Digital Object Identifier):
Page No: a546-a558
Country: -, -, India .
Area: Engineering
ISSN Number: 2349-5162
Publisher: IJ Publication
Published Paper URL :: https://www.jetir.org/view?paper=JETIR2105064
Published Paper PDF: https://www.jetir.org/papers/JETIR2105064

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