• Sudharsan K
• Dr. R. Mahenthiran

Abstract: Shigella flexneri is the main agents of bacillary dysentery. Shigellosis is a common bacterial disease is mainly transmitted through the faecal–oral route. Chicken IgY has been identified as an alternative for the treatment of Shigellosis. 21 weeks old white leghorn chicken which lays eggs was immunized with Shigella flexneri. The anti-Candida albicans IgY was purified and their protein content was estimated. The specificity of produced Serum IgY and egg yolk IgY was tested with Rapid Agglutination test. SDS-PAGE analysis of IgY showed a protein profile of the IgY, antibody titre potency of each serum IgY and egg yolk IgY was determined by Indirect ELISA. Collection and Preparation of leaf extract of Rhododendron nilagiricum. To evaluate the Antibacterial and Phytochemical analysis of leaf extract. The present study was undertaken to generate antibodies against Shigella flexneri which cause Diarrheal infection and to examine the growth inhibitory activity of the Shigella flexneri by antibodies produced at different concentration for both IgY antibody in Egg Yolk and serum. Then also as a comparative counterpart the methanol extract of the desired plant is taken. This extract was taken in different concentration and was also assayed for the growth inhibitory activity.

Keywords: Shigella flexneri, IgY, ELISA, Rhododendron nilagiricum, Growth inhibitory Assay.

"Generation and Characterization of chicken egg yolk antibodies against Shigella sp and comparison studies with Rhododendron nilagiricum.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 5, page no.d130-d138, May-2021, Available :http://www.jetir.org/papers/JETIR2105401.pdf

"Generation and Characterization of chicken egg yolk antibodies against Shigella sp and comparison studies with Rhododendron nilagiricum.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 5, page no. ppd130-d138, May-2021, Available at : http://www.jetir.org/papers/JETIR2105401.pdf