UGC Approved Journal no 63975(19)

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Published in:

Volume 8 Issue 6
June-2021
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2106801


Registration ID:
311583

Page Number

f722-f737

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Title

Advances of transcriptomics in crop improvement: A Review

Abstract

The transcriptome defines the whole set of transcripts available in a cell for a specific point of time and condition. Interpreting these functional elements of the genome is key to reveal the phenotype or response of an individual or cell. Broadly, to understand the physiological and molecular events in terms of genome sequence, gene differentiation, gene expression regulation, posttranscriptional modifications, editing and gene splicing, transcriptome study is essential to answer the fundamental biological queries. Over the years, to deduce and quantify the transcriptome there have been a drastic shift from simple PCR (RT-PCR)/hybridization (Northern blot) dependent candidate gene-based detection to random end sequencing-based (tag-based; ESTs, SAGE, MPSS) approaches followed by more recent, complicated genome-wide high throughput global expression profiling (Microarray & sequencing based). The advancement of next generation sequencing platforms has revolutionized not only the knowledge of dynamic transcriptomics but also provides multi-dimensional analysis in terms of novel transcript discovery, structural detection at single base resolution, functional annotation, mapping, differential gene expression, enrichment analysis etc. Furthermore, cDNA based and tag-based sequencing approaches are not amenable for short/degraded RNAs or non-coding RNAs, and for those, RNA-seq or single molecule direct RNA sequencing have been developed to overcome these limitations. Transcriptomics in recent years have vast applicability in every aspect in the field of agriculture, medicine, environment for overall strategic improvements. In near future, enough scope is there to introduce more advance technologies to bridge the gap of recent technological shortcomings.

Key Words

Next Generation Sequencing (NGS), transcriptome, e-QTL, RNA-seq

Cite This Article

"Advances of transcriptomics in crop improvement: A Review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 6, page no.f722-f737, June-2021, Available :http://www.jetir.org/papers/JETIR2106801.pdf

ISSN


2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"Advances of transcriptomics in crop improvement: A Review", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 6, page no. ppf722-f737, June-2021, Available at : http://www.jetir.org/papers/JETIR2106801.pdf

Publication Details

Published Paper ID: JETIR2106801
Registration ID: 311583
Published In: Volume 8 | Issue 6 | Year June-2021
DOI (Digital Object Identifier): http://doi.one/10.1729/Journal.27477
Page No: f722-f737
Country: Almora, Uttarakhand, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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