• PASUPATI NATH TIWARI
• Ayesha Rehman
• Dr. C. Sreedhar
• Ram Autar Bishwakarma

A simple, accurate, precise and sensitive reverse phase high performance liquid chromatography method was developed for estimation of Rifapentine in bulk and pharmaceutical dosage form. For HPLC method, Column: C18 (4.6ID x 250mm) in isocratic mode with mobile phase containing Acetonitrile: 0.01M KH2PO4 buffer pH (6.0) in ratio of 80: 20 v/v was used. The flow rate was 0.8 ml/min with injection volume of 20µl and effluent was monitored at 478nm. Retention time was found to be 5.00 ± 0.1 minute. A simple, precise and economical UV-Spectrophotometric method has been established for the quantification of Rifapentine in bulk drug and tablets. In this method Area under Curve (AUC) was integrated in the wavelength range of 307 -350 nm. Rifapentine obeyed linearity in the concentration range of 4 - 24 µg/mL with r 2> 0.9994

Rifapentine, RP-HPLC, Area under- curve, UV-Spectrophometry, Method

"METHOD DEVELOPMENT, VALIDATION, AND ESTIMATION OF RIFAPENTINE IN BULK AND TABLET DOSAGE FORM BY USING UV-SPECTROPHOTOMETRY AND RP-HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 8, page no.a109-a123, August-2021, Available :http://www.jetir.org/papers/JETIR2108016.pdf

"METHOD DEVELOPMENT, VALIDATION, AND ESTIMATION OF RIFAPENTINE IN BULK AND TABLET DOSAGE FORM BY USING UV-SPECTROPHOTOMETRY AND RP-HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 8, page no. ppa109-a123, August-2021, Available at : http://www.jetir.org/papers/JETIR2108016.pdf