• Dr. Dinesh Prakashrao Kawade
• Bhadke M.Y.
• Asnani A. J.
• Chaple D. R.

The present research work was carried out with a series of Mannich bases of substituted benzimidazole and their derivatives were prepared by both conventional heating and microwave assisted techniques. Benzimidazole and their derivatives were prepared through condensation between o-phenylenediamine and six different substituted aliphatic acids in presence of hydrochloric acid, then the product undergone Mannich reaction in presence of formaldehyde, ethanol, primary amines and ethanol to obtained six different substituted Mannich bases of benzimidazole derivatives. All the synthesized compounds were subjected to TLC to find out the purity. The synthesized derivatives were characterized by spectroscopic data and were evaluated for antimicrobial activity and antioxidant activity by using Cup plate method and DPPH free radical scavenging assay respectively. The results of antimicrobial activity (in-vitro) revealed that the compounds (2a, 2b, 2c & 2f) possessed significant antimicrobial activity. Among the compound tested, the compound (2d) compound (2d) had shown the highest antibacterial & antifungal activity which was comparable to that of ciprofloxacin and fluconazole respectively. The substituent of amino group on benzimidazole ring contributed significantly towards amino acid. This study suggested that microwave assisted method can be appropriate for the synthesis of benzimidazole and their derivatives with better purity, yield and ecofriendly method.

Benzimidazole, Mannich bases, Conventional synthesis and Microwave assisted method, Antimicrobial activity and Anti-oxidant activity

"SYNTHESIS AND BIOLOGICAL EVALUATIONS OF SOME NOVEL MANNICH BASES OF BENZIMIDAZOLE AND THEIR DERIVATIVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 8, page no.b292-b305, August-2021, Available :http://www.jetir.org/papers/JETIR2108167.pdf

"SYNTHESIS AND BIOLOGICAL EVALUATIONS OF SOME NOVEL MANNICH BASES OF BENZIMIDAZOLE AND THEIR DERIVATIVES", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 8, page no. ppb292-b305, August-2021, Available at : http://www.jetir.org/papers/JETIR2108167.pdf