• Nayan pardeshi
• H.S Bhawar
• S.D Magar

The present study describes a simple, accurate, precise and cost-effective reverse phase High Performance Liquid Chromatographic method for estimation of vinpocetine in their pharmaceutical dosage form. The separation was carried on Kromasil, C18, 250 mm X 4.6 mm, 5 µm. Detection was done using UV detector at isocratic point 228 nm. The developed method employed mobile Acetonitrile: Buffer (90:10 % v/v), with flow rate 1.0 ml/min. High linearity of the developed method was confirmed over concentration range 5-75 µg/ml for vinpocetine with the correlation coefficient of 0.999. The Percentage RSD for precision of the method was found to be less than 2%. The percentage recoveries for vinpocetine were found to be in range 98.00-102.00 w/v. Peaks was obtained at retention time 6.4 min for Vinpocetine. By using all the above parameters, a simple, accurate, precise and cost-effective method were developed, optimize and validate. Some method by HPLC and UV, FTIR are already reported in vinpocetin drug .

RP-HPLC, Method Optimization, development, Validation, vinpocetine.

"“DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF VINPOCETINE IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 8, page no.b971-b993, August-2021, Available :http://www.jetir.org/papers/JETIR2108250.pdf

"“DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF VINPOCETINE IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 8, page no. ppb971-b993, August-2021, Available at : http://www.jetir.org/papers/JETIR2108250.pdf