• Biswajit Saha
• Ankita Samanta
• Ankit Kumar Nath

Every year the manufacturing of X-ray film sheets worldwide can use up to 1000 tons of total silver wasted using these chemically manufactured films. To avoid waste and promote re-use of the plates, biological methods are used to study the effect of disposed X-rays on the production of two types of enzymes using the power of microorganisms. The biological agents like a series of fungi may be used so that isolation of the protease and usage of it may be shown. The dumping ground was located with an area that had pretty huge reserves of X-ray films and other medical items. Proper care was taken in handling and separation of the soil from the regions that were demarcated from the five different sites of the NDDM ground. The isolates were collected and checked for protease activity and then isolates that showed best results were marked as T1 and T2. First, the production of the protease enzyme following the use of disposed X-rays as a carbon source. Secondly, nitrate reductase enzyme may help in the reduction of the silver nitrates that are present in the X-Ray films. All discarded X-rays are brought into the substrate. Different fungal isolates were collected from the region around the North Dum Dum Municipality Dumping Ground. The different amounts of the isolates were taken and they were utilized in the making of the experimental models that used to verify the hypothesis and untapped or unconventional methods of using the fungi as potential sources cleaning x-ray films naturally. The isolated enzyme was then used for further series of experiments, and the hypothesis regarding the usage of the fungi was used and verified, the experiments were conducted in duplicate sets for confirmation.

Bioremediation, X ray, Nitrate reductase, Fungi, Protease

"Isolation And Characterization Of Protease Producing Fungi For X-Ray Plate Bioremediation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 8, page no.c968-c975, August-2021, Available :http://www.jetir.org/papers/JETIR2108369.pdf

"Isolation And Characterization Of Protease Producing Fungi For X-Ray Plate Bioremediation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 8, page no. ppc968-c975, August-2021, Available at : http://www.jetir.org/papers/JETIR2108369.pdf