• Dr Athira VJ

ABSTRACT Background: The 80% of patients with diabetes mellitus die a thrombotic death. A blood clot (thrombus) developed in the circulatory system can cause vascular blockage leading to serious consequences including death. Objectives: To estimate the serum protein study and DNA pattern in normal individual and diabetic patients by using following parameters, Protein profiling SDS PAGE (sodium dodecayl sulphate poly acryl amide gel electrophoresis),DNA study,PCR (polymerase chain reaction),RFLP (Restriction Fragment length polymorphism Secondary objectives were To find out the thrombolytic activity of streptokinase enzyme in DM patients presented with cardiovascular diseases Materials & methods: The study was carried out in 11samples of Diabetic mellitus patients. Samples were collected and processed in centre for Bioscience and Nanoscience Research the within a period of 2 months (October 2015– December 2015). The samples were collected & processed by standard biochemical technique.such as comparative study of albumin / globulin (A/G) ratio,protein ,albumin,and globulin in DM and normal individual were done. Determination of molecular weight of protein by Sodium Dodecyl sulphate- Polyacrylamide Gel Electrophoresis.and DNA study also included,the study of streptokinase enzyme were done. Result: Eleven diabetic patients were selected for study. By using significant testing pattern used to achieve satisfactory results DNA was isolated and its pattern was studied using agarose gel electrophoresis. The serum protein pattern study was also conducted. Using RFLP method for separating this. SDS PAGE is a powerful technology that can detect and identify the changes of the structure and function of proteins in response to intra- and extracellular environmental signals or disease states. As proteomics (Proteomics is a large-scale, comprehensive study of the proteins of a cell or organism. It is a unique means of characterizing proteins that are expressed in a cell or tissue at any given time-point and of identifying any modifications that they may undergo.) Can establish a link for genes and proteins with a disease, it will play an important role in defining the molecular determinants of a disease and in identifying targets for drug discoveries and diagnostics. We have carried out proteomics studies in sample that are obtained from diabetic patient that are related to cardio vascular diseases and also investigate the significant fibrinolytic role of streptokinase enzyme in cardio vascular disease.

:DM,RFLP,PCR,SDS PAGE

"“STUDY OF SERUM PROTEINS AND DNA PATTERN OF DIABETIC AND NON-DIABETIC SERUM SAMPLES WITH THROMBOLYTIC ACTIVITY OF STREPTOKINASE ENZYME”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 9, page no.e7-e66, September-2021, Available :http://www.jetir.org/papers/JETIR2109402.pdf

"“STUDY OF SERUM PROTEINS AND DNA PATTERN OF DIABETIC AND NON-DIABETIC SERUM SAMPLES WITH THROMBOLYTIC ACTIVITY OF STREPTOKINASE ENZYME”", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 9, page no. ppe7-e66, September-2021, Available at : http://www.jetir.org/papers/JETIR2109402.pdf