• Sandeep Paney
• Arvind Singh

Selaginella is a cosmopolitan genus of small spreading, herbaceous spore-bearing vascular plants. Selaginella, an extant genus of primitive vascular plants, has survived over 400 million years of evolution. In vitro morphogenesis in Selaginella is considered for the first time to establish a well documented aseptic culture on half- strength Murashige and Skoog’s basal medium with 2ip (4.92—49.21 M), or Kn (4.65—46.47 M) or GA3 (2.89—28.90 M) for shoot multiplication, and with different concentrations of IBA (4.9—49 m) to initiate root cultures. GA3 was instrumental for shoot multiplication as well as induction of reproductive structures in each and every leaf axil. On the other hand, it is observed that IBA alone in Selaginella can act as signal molecules for induction of enormous numbers of root masses from a few existing roots. An interesting pattern of re-differentiation has also been observed where apical portions of large numbers of roots were converted to green shoot apical meristems. Further differentiation produced tiny green shoots. Distinct bipolarity was noted in shoots when they were isolated from root masses and appeared as embryo-like structures. Chromosome analysis from in vitro sporophytic plants revealed 2n=16 chromosomes, indicating chromosomal stability. The interesting in vitro pattern of morphogenesis obtained in Selaginella may provide new insights into totipotency of plants.

Selaginella, Multiplication, Re-differentiation

"Morphogentical Study of Selaginella selaginoides Spring", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.0, Issue 0, page no.0, December-2021, Available :http://www.jetir.org/papers/JETIR2110118.pdf

"Morphogentical Study of Selaginella selaginoides Spring", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.0, Issue 0, page no. pp0, December-2021, Available at : http://www.jetir.org/papers/JETIR2110118.pdf