• Sonal sareen Pathak

Abstract: The present investigation was conducted to study effect of mutagenesis for increased glucoamylase productivity by local soil fungi of Jabalpur region. The fungus was subjected to mutagenesis using ultraviolet radiation, nitrous acid and ethidium bromide treatment for increased glucoamylase production. After the treatment and 7 repeated subculture, expression of enzyme activity by the mutant SSP#16NA3 was found to be irrevocable. The glucoamylase production in soild state fermentation by mutant isolate (SSP#16NA3 mutant) was done in solid substrate (rice bran) in standard basal medium at temperature 27°C with pH 6.0 and incubated for 96 h. The potent mutant isolate showed nearly 3 fold increase in enzyme activity as compared to wild strain. This local fungal isolate showing enhanced glucoamylase activity can be exploited for achieving economy in starch processing industries.

Keyterms: mutagenesis, ultraviolet radiation, nitrous acid, ethidium bromide, soild state fermentation, mutant isolate

"EFFECT OF MUTAGENS ON LOCAL FUNGAL ISOLATE OF JABALPUR REGION FOR OVER-EXPRESSION OF GLUCOAMYLASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 12, page no.b652-b659, December-2021, Available :http://www.jetir.org/papers/JETIR2112175.pdf

"EFFECT OF MUTAGENS ON LOCAL FUNGAL ISOLATE OF JABALPUR REGION FOR OVER-EXPRESSION OF GLUCOAMYLASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 12, page no. ppb652-b659, December-2021, Available at : http://www.jetir.org/papers/JETIR2112175.pdf