UGC Approved Journal no 63975(19)

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Published in:

Volume 8 Issue 12
December-2021
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2112186


Registration ID:
317741

Page Number

b732-b746

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Title

In Vitro Antioxidant and Anticancer Activity of Andrographis serpyllifolia (Rottler Ex Vahl) Wight on Human Cancer Cell Lines.

Abstract

Andrographis serpyllifolia (Rottler ex Vahl) Wight (Acanthaceae) is the plant which found in South India in the state of Karnataka, Tamil Nadu and Andhra Pradesh shows various biological activities. Whole plant subjected to extraction and followed by fraction. Phytochemical investigation was carried out using different biochemical tests. Total phenolics in extract and fractions were determined quantitatively by Folin-Ciocalteau reagent method. The antioxidant activity was evaluated by Diphenyl Picryl Hydrazyl (DPPH) radical scavenging method, nitric oxide radical inhibition method, reducing power method. In vitro anticancer activity on MCF-7, A549, and HEK 293 cancer cell lines was carried out by (3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. The Phytochemical investigation confirmed the presence of alkaloids, glycosides, phenolic compounds, flavonoids, tannins, sterols, saponins, fixed oils and fats, protein and amino acids, Gum and mucilage, and carbohydrates. Ethanol extract followed by ethyl acetate fraction were shown highest antioxidant activity. The ethanol extract significantly reduced the percent viability of MCF-7, A549 and HEK 293 cancer cell lines with IC50 value 410 ± 11.547 µg/ml for MCF-7, 428.34 ± 10.137 µg/ml for A549 and 426.67 ± 8.819 µg/ml for HEK 293.

Key Words

A. serpyllifolia, phytochemical, antioxidant, anticancer, cancer cell lines

Cite This Article

"In Vitro Antioxidant and Anticancer Activity of Andrographis serpyllifolia (Rottler Ex Vahl) Wight on Human Cancer Cell Lines.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 12, page no.b732-b746, December-2021, Available :http://www.jetir.org/papers/JETIR2112186.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"In Vitro Antioxidant and Anticancer Activity of Andrographis serpyllifolia (Rottler Ex Vahl) Wight on Human Cancer Cell Lines.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 12, page no. ppb732-b746, December-2021, Available at : http://www.jetir.org/papers/JETIR2112186.pdf

Publication Details

Published Paper ID: JETIR2112186
Registration ID: 317741
Published In: Volume 8 | Issue 12 | Year December-2021
DOI (Digital Object Identifier):
Page No: b732-b746
Country: Wardha, Maharashra, India .
Area: Pharmacy
ISSN Number: 2349-5162
Publisher: IJ Publication


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