• Sidra urooj
• Dr. Iffath rizwana

Abstract: Aim and objective: The purpose of this research work was to Develop and validate a sensitive and accurate stability indicating RP-HPLC method for the estimation of Amoxicillin, Omeprazole and Rifabutin in bulk and formulation and its applications in dissolution studies. Methods: Separation of ALN, RBN & OMP was conceded using 5.0 µm C18, Waters column (at 27 °C), with 60:40 vol/vol mix ratio, NaHSO4 (0.1M, pH 4.5) & methanol like mobile phase and movement rate of 1.0 ml/min, sensed at 246 nm. Results: Retention times of ACN, RFN and OPE were 2.122 min, 2.844 and 3.93 min, respectively. Method linearity scope was ranged from 125 – 375 μg/ml for ACN, 5 – 15 μg/ml for OPE and 6.25 – 18.75 μg/ml for RFN. The accuracy was computed in the range of 98.13–101.07% and the precision was between 0.282% and 0.569% relative standard deviation for three drugs. The method can effectively separate the degradation products from ACN, RFN and OPE. Key words- RP-HPLC, amoxicillin, omeprazole, rifabutin, dissolution studies.

Key words- RP-HPLC, amoxicillin, omeprazole, rifabutin, dissolution studies.

"Development and validation of stability indicating rp-hplc method for the estimation of amoxicillin, omeprazole and rifabutin in bulk and formulation and its applications in dissolution studies ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.8, Issue 12, page no.c354-c364, December-2021, Available :http://www.jetir.org/papers/JETIR2112246.pdf

"Development and validation of stability indicating rp-hplc method for the estimation of amoxicillin, omeprazole and rifabutin in bulk and formulation and its applications in dissolution studies ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.8, Issue 12, page no. ppc354-c364, December-2021, Available at : http://www.jetir.org/papers/JETIR2112246.pdf