• Dipika Chavda
• Atindra Shukla
• Tejal Soni

ABSTRACT Background: The cost-effective, simple and accurate method for simultaneous estimation of drugs is high-performance thin-layer chromatography (HPTLC). In this research, Naproxen and Pomegranate Peel Extract (PPE) was measured simultaneously by the HPTLC method. Methods: The stationary phase was Precoated silica gel G60 F254 aluminium sheets (10 x 10 cm) while mobile phase was ethyl acetate: toluene: methanol(4:5.5:0.5 v/v/v). The chromatographic analysis was performed in reflection or absorbance mode at 240 nm. The validation of the developed method included linearity, the limit of detection, the limit of quantification, accuracy, precision, specificity and robustness as per ICH Q2 (R1) guidelines. Results: The Rf value of Naproxen was 0.52 ± 0.01 and for Pomegranate Peel Extract was 0.83 ± 0.01. The linear regression analysis of both drugs showed a linear relationship between 200-1000 ng/band. The regression equation of Naproxen was y = 3.5847x + 253.22 with 0.9953 correlation coefficient, while regression equation of PPE was y = 3.5371x - 124.28 with 0.9928 correlation coefficient. Conclusion: The method was accurate and precise, which can further be used to estimate the drugs simultaneously.

Keywords: Pomegranate Peel Extract, Naproxen, high-performance thin-layer chromatography, simultaneous estimation, validation.

"HPTLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NAPROXEN AND POMEGRANATE PEEL EXTRACT IN COMBINED DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 1, page no.a70-a80, January-2022, Available :http://www.jetir.org/papers/JETIR2201009.pdf

"HPTLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF NAPROXEN AND POMEGRANATE PEEL EXTRACT IN COMBINED DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 1, page no. ppa70-a80, January-2022, Available at : http://www.jetir.org/papers/JETIR2201009.pdf