• Dr. Keshamma E
• Dr. Jyothsna B. S.
• Kavitha K. R.

To study the anatomical features of terrestrial orchid (Nervilia crociformis) collected from natural habitats of western ghats, Karnataka, India. The terrestrial orchid, Nervilia crociformis, was collected from natural habitats from various locations of Kemmangundi and Sagar, Karnataka State. Freshly collected root/tuber of Nervilia crociformis was used for anatomical studies. Free hand sections were taken, stained with tryphan blue and viewed under the light microscope. Ultra-thin sections of roots of Nervilia crociformis were taken and viewed under transmission electron microscopy (TEM) and the colonized cells was studied. Light microscopic study revealed varied distribution of intact and lysed pelotons in the root cortex and the tubers were sparsely colonized. The extent of peloton formation was assessed in all the species. TEM studies Nervilia crociformis revealed fine structure of pelotonic hyphae, their lysis and interfacial membrane. Anatomical studies of Nervilia crociformis by light and transmission microscopy revealed the presence of mycorrhizal association.

Nervilia crociformis, Anatomical studies, Light microscopy, TEM, Mycorrhizal association

"Anatomical Studies of Nervilia crociformis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 1, page no.a270-a275, January-2022, Available :http://www.jetir.org/papers/JETIR2201035.pdf

"Anatomical Studies of Nervilia crociformis", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 1, page no. ppa270-a275, January-2022, Available at : http://www.jetir.org/papers/JETIR2201035.pdf