• Hardik Giri Gosai
• Swati Narolkar

A bioflocculant producing slightly mucoid bacterium Bacillus species (BF9) has been isolated from shrimp pond water. This study represents the isolation of bioflocculant producing microorganisms from diverse sources. Three isolates BF6, BF8, and BF9 shows bioflocculant activity out of 20 studied samples, among them BF9 represented the highest bioflocculant activity and was selected for subsequent study. Isolate BF9's protein and carbohydrate composition has been determined. Various bioflocculant concerning parameters such as carbon, nitrogen, pH, temperature, and cation has been optimized by utilizing kaolin as suspended particle via flocculation assay. Glucose and ammonium chloride are confirmed to be a good sources of carbon and nitrogen. Among the other cations, ferric chloride had a significant influence, enhancing the flocculation rate of BF9 by 83%. At pH 8, isolate BF9 had an 86.24% flocculation activity. At a temperature of 30°C, the flocculation activity of BF9 was reported to be 78.8%. The optical density of flocculating activity has been measured using a Kaolin clay assay.

Bioflocculant, Extracellular Polymeric Substances, Flocculation, Shrimp Pond Water

"Isolation, Characterization and Optimization of Bioflocculant Producing Bacteria from the Aquaculture Ponds ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 1, page no.a434-a442, January-2022, Available :http://www.jetir.org/papers/JETIR2201059.pdf

"Isolation, Characterization and Optimization of Bioflocculant Producing Bacteria from the Aquaculture Ponds ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 1, page no. ppa434-a442, January-2022, Available at : http://www.jetir.org/papers/JETIR2201059.pdf