• P. Venkateswara Rao
• Mohinilatha Katuri

The present paper describes a simple, accurate and precise reversed phase High Performance Liquid Chromatography (HPLC) method for rapid and simultaneous quantification of Tivozanib. The chromatographic separation was achieved on Symmetry C18 (150x4.6mm, 3.5µm) column. Mobile phase contained a mixture of Hexane sulphonic acid and Acetonitrile in the ratio of 40:60 v/v, flow rate 1.0ml/min and UV detection at 226nm. The proposed method shows a good linearity in the concentration range of 20-120µg/ml of Tivozanib under optimized conditions. Precision and recovery study results are in between 98-102%. In the entire robustness conditions %RSD is below 2.0%. Degradation has minimum effect in stress condition and solutions are stable up to 24hrs. This method is validated for different parameters like Precision, linearity, accuracy, limit of detection (LOD), limit of quantification (LOQ), ruggedness, robustness and forced degradation study were determined according to the ICH Q2B guidelines. All the parameters of validation were found to be within the acceptance range of ICH guidelines.

RP-HPLC, Validation, Tivozanib

"A NEW METHOD FOR THE ESTIMATION OF TIVOZANIB IN PHARMACEUTICAL FORMULATION BY USING RP-HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 1, page no.a586-a591, January-2022, Available :http://www.jetir.org/papers/JETIR2201078.pdf

"A NEW METHOD FOR THE ESTIMATION OF TIVOZANIB IN PHARMACEUTICAL FORMULATION BY USING RP-HPLC METHOD", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 1, page no. ppa586-a591, January-2022, Available at : http://www.jetir.org/papers/JETIR2201078.pdf