• Srinivasa Rao N
• Gugulothu Jyothi

An easy, quick, precise, active and reproducible LC-MS/MS technique was developed for the bio analytical method of Oritavancin with D6-Oritavancin as internal standard. Separation was carried on X-Bridge phenyl column (250 mm x 4.6mm, 5µm) using a isocratic elution with a buffer containing 0.1% Tri fluoro acetic acid and Acetonitrile in the ratio of 40:60 as mobile phase with 1mL/min flow rate at ambient temperature . Analysis was carried out within 3 minutes over a good linear concentration range from 26 ng/mL to 204 ng/mL (r2 = 0.9996) for Oritavancin. Precision and recovery study results are within the acceptable limit. This method has been successfully applied, exploring Oritavancin with its internal standard (D6-Oritavancin) was extracted from rat plasma using liquid -liquid extraction. This strategy was applied for Freeze thaw, auto sampler, bench top and long-term stability studies, we found that the drugs were stable throughout the stability studies according to USFDA guidelines.

LC-MS/MS, Oritavancin, Rabbit plasma

"NEW BIOANALYTICAL METHOD FOR THE ESTIMATION OF ORITAVANCIN IN RABBIT PLASMA BY USING LCMS/MS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 1, page no.f39-f44, January-2022, Available :http://www.jetir.org/papers/JETIR2201508.pdf

"NEW BIOANALYTICAL METHOD FOR THE ESTIMATION OF ORITAVANCIN IN RABBIT PLASMA BY USING LCMS/MS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 1, page no. ppf39-f44, January-2022, Available at : http://www.jetir.org/papers/JETIR2201508.pdf