• ASHWINI P. ALEGAONKAR
• VARSHA S. HONMORE

Graphene oxide (GO) is recognized as a potential candidate for the biomedical applications. We report, preparation and use of GO to test its response against Escherichia coli (E-coli), and Staphylococcus aureus (S-aureus) bacteria. Initially, GO is synthesized by modified Hummer’s technique, reprecipitated at different pH (@7,10,12, 14) and tested for its antibacterial response, using the diffusion methodology. The HRTEM images shows for GO@7, is extremely transparent showing maximum exfoliation of the conjugated graphitic layers and same is reflected in Raman and intensity of 2D band is remarkably prominent. Notably, GO pH@7 showed maximum efficacy thereby inhibiting more gram-negative bacteria with compared to gram positive. GO pH@7. Further, tests using phosphate buffer that showed total inactivity towards both the bacterial strains. By adjusting pH, externally, GO showed antibacterial activity more than internally adjusted pH for GO. The calculated MIC value for this is 200 µg/mL external buffer.

GO at different pH values, antibacterial activity

"Synthesis of graphene oxide at variable pH and its response to antibacterial activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.b219-b231, February-2022, Available :http://www.jetir.org/papers/JETIR2202127.pdf

"Synthesis of graphene oxide at variable pH and its response to antibacterial activity", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppb219-b231, February-2022, Available at : http://www.jetir.org/papers/JETIR2202127.pdf