• Pramod Kumar Singh
• Sunita Singh
• Aman Saifi
• Shalini G Pratap

Enzymes act as biocatalyst which has several advantages over chemical catalysts due to their eco-friendly nature that make them a favorable choice for almost all industrial applications. The present study aims to assess the destaining properties of protease enzymes purified from three different plant sources i.e., jasmine flower (Jasminum sambac), ginger rhizome (Zingiber officinale) and banana leaf (Musa paradisiaca). Various parameters were determined like plant protein content, protease activity, specific activity, optimal temperature, pH and kinetics of protease enzyme. The result indicated that the jasmine flower showed the maximum protein content and protease activity followed by ginger rhizome and banana leaf. The optimal temperature for the activity of protease enzyme was observed between 70°C and 100°C in ginger rhizome while around 40°C in jasmine flower and banana leaf. The highest protease enzyme activity was observed at pH 7 in jasmine flower and ginger rhizome while at pH 6 in banana leaves. The kinetics indicated that maximum Vmax (3.27 µM/sec) was observed in jasmine flower followed by ginger rhizome and banana leaf which values were 2.405µM/sec and 1.628 µM/sec respectively. The maximum Km value of banana leaf was 0.0091 mM/l followed by jasmine (0.003 mM/l) and ginger rhizome (0.00531 mM/l). The specific activity of partially purified extract of ginger rhizome and jasmine flower was highest (at 60% salt saturation) while it was found 90% in banana leaf. Based on their efficient protease activity, crude enzymes partially purified from different sources were competent in removing the different stains such as roli, coffee, turmeric, tea, and ink from the stained cloth. Hence, these plants can be a potent candidate for enzyme sources as an additive in the large-scale detergent industry.

Protein, protease enzyme, kinetics, destaining, stain, plant source

"Comparative destaining properties of protease enzyme extracted from jasmine (Jasminum sambac), banana (Musa paradisiacal) and ginger (Zingiber officinale) ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.b515-b519, February-2022, Available :http://www.jetir.org/papers/JETIR2202160.pdf

"Comparative destaining properties of protease enzyme extracted from jasmine (Jasminum sambac), banana (Musa paradisiacal) and ginger (Zingiber officinale) ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppb515-b519, February-2022, Available at : http://www.jetir.org/papers/JETIR2202160.pdf