• Mrinalini C. Damle
• Pranav S. Uttekar
• Akshay H. Punmiya
• Aishwarya A. Mate

A combination of sofosbuvir and velpatasvir is available in the market for treatment of Hepatitis C viral infections. It has advantage of once daily dose. A new simple derivative spectrophotometric method has been developed and validated for simultaneous estimation of sofosbuvir and velpatasvir. Quantitative estimation was carried out at 260nm and 269 nm for velpatasvir and sofosbuvir respectively, in first derivative mode. The calibration curves were linear over a concentration range of 3-15µg/ml for velpatasvir and 12-60µg/ml for sofosbuvir. Method validation was assessed as per the ICH guidelines Q2(R1). The developed method was successfully applied for analysis of these two drugs in a mixture and excellent recovery results were obtained. The method is simple, fast and economic. The method can be applied to the quality control testing of sofosbuvir and velpatasvir in their combination.

Sofosbuvir, Velpatasvir, UV derivative spectrophotometry.

"DETERMINATION OF SOFOSBUVIR AND VELPATASVIR IN COMBINATION BY DERIVATIVE UV SPECTROPHOTOMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.c94-c99, February-2022, Available :http://www.jetir.org/papers/JETIR2202232.pdf

"DETERMINATION OF SOFOSBUVIR AND VELPATASVIR IN COMBINATION BY DERIVATIVE UV SPECTROPHOTOMETRY", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppc94-c99, February-2022, Available at : http://www.jetir.org/papers/JETIR2202232.pdf