• Ashok S
• Rajakumar S
• Ayyasamy PM

Objectives: To Isolate the Lactic Acid Bacteria, Produce the bacteriocine and teste the antimicrobial activity. Methods: Bacteriocin producing lactic acid bacteria (LAB) was isolated from raw milk and cheese samples using de Man, Rogosa and Sharpe (MRS) plate. The isolated LAB was identified by morphological and biochemical tests and confirmed as Streptococcus sp. and Lactobacillus sp. Supernatant of Streptococcus and Lactobacillus culture are partially purified and used for bactericidal activity test against clinical pathogens such as Bacillus, Pseudomonas, Staphylococcus, and Salmonella. Results: The antimicrobial activity test performed and among the 4 pathogens Lactobacillus were effectively inhibited clinical pathogens and showed higher inhibitory activity against Staphylococcus and Pseudomonas. The Streptococcus and Lactobacillus were analyzed by SDS-PAGE (10%) polyacyrlamide slab to separate the proteins. The presence of protein band nearing the molecular weight around 75 kda confirmed the presence of bacteriocin. Conclution: The bacteriocins produced from milk products is the very good probiotic natural food. The peculiar antimicrobial activity of Lactobacillus and Streptococcus can positively have influence on their use as appetiser cultures for modern fermented nutriments and natural antibiotics.

Bacteriocin, Lactic acid bacteria, Bactericidal activity, Streptococcus, Lactobacillus,

"BACTERIOCIN PRODUCTION AND BACTERICIDAL ACTIVITY AGAINST CLINICAL PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 2, page no.d198-d204, February-2022, Available :http://www.jetir.org/papers/JETIR2202326.pdf

"BACTERIOCIN PRODUCTION AND BACTERICIDAL ACTIVITY AGAINST CLINICAL PATHOGENS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 2, page no. ppd198-d204, February-2022, Available at : http://www.jetir.org/papers/JETIR2202326.pdf