• Arun A. Shetti
• Neharu K.
• Geetanjali Kamble
• DR. S. V. Hermath

ABSTRACT L-asparaginases are the therapeutic proteins and are accounted for around 40 % of anti-leukemic and antilymphoma agents it is also used in the design of biosensors a diagnostic biosensor. The soil samples were collected from different locations around Hubli city. The soil isolates showing potential L-asparaginase activity were identified and characterized. The partially purified L-asparaginase form soil isolate Bacillus Sp. showed a specific activity of 6.0IU/mg. The L-asparaginase isolated showed maximum activity at 370c with glucose and beef extract as carbon and nitrogen source respectively. The L-asparaginase for soil isolate Bacillus sp. Can be utilized as commercially viable L-asparaginase enzyme to be used in pharmaceutical and food industry. Keywords: L-asparaginases, soil Isolates, Bacillus sp.

Keywords: L-asparaginases, soil Isolates, Bacillus sp.

"Isolation and Characterization of L-asparaginase isolated from soil isolate Bacillus sp.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 3, page no.a318-a327, March-2022, Available :http://www.jetir.org/papers/JETIR2203038.pdf

"Isolation and Characterization of L-asparaginase isolated from soil isolate Bacillus sp.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 3, page no. ppa318-a327, March-2022, Available at : http://www.jetir.org/papers/JETIR2203038.pdf