• R B Hosamani
• Dr. S S Sarawad

Background: Herbal plants are being used for the treatment of various ailments in the Ayurvedic system of Medicine. Ayurveda mentioned various plants and their compound formulations for the treatment of polluted and contaminated water. It has been estimated that up to 80% of all diseases and sicknesses in many developing countries are caused by inadequate sanitation, polluted and contaminated water or unavailability of water. The present study aims to analyze the phytochemical and antibacterial activity of Dhavādi Bhasma and its applications in water purification as a natural herbal coagulant. Methods: Dhavādi Bhasma was analysed for antibacterial activity against six different microbes of standard bacterial strains by using MIC & MBC methods. Three different water samples such as Ghataprabha River (WS-1), Muchakhandi Kere (Lake) (WS-2) and Pond (WS-3) were collected from Bagalkot city. All the samples were treated with Dhavādi Bhasma at the optimum dosage of 3gm/l by jar test method to assess the coagulation potential. The untreated and treated water samples were analysed for physicochemical and microbiological parameters by using the standard methods. Results: Dhavādi Bhasma showed antibacterial activity against tested six different microbes of standard bacterial strains i.e., Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa. Treatment of 3gm/l of Dhavādi Bhasma significantly reduced the water turbidity thereby achieving a turbidity reduction of 91.26%, 90.57% and 91.90% for the Ghataprabha River (WS-1), Muchakhandi Kere (Lake) (WS-2) and pond water (WS-3) samples respectively. However, the resultant pH, conductivity, alkalinity, TDS and Total hardness were partially increased but remained within the permissible limit as per WHO/USPH standards. A significant reduction in the microbial load (coliform counts) was found with the water of the Ghataprabha River and pond water (71.42%) and Muchakhandi Kere (66.66%). Conclusion: Dhavādi Bhasma had strong antibacterial activity against tested six different microbes of standard bacterial strains. It acts as an efficient natural herbal coagulant and anti-bacterial agent which makes the Dhavādi Bhasma a good source for water purification.

Dhavādi Bhasma, River water treatment, Antibacterial activity, Natural coagulant

"AN IN-VITRO STUDY ON ANTI-BACTERIAL ACTION OF DHAVĀDI BHASMA IN WATER PURIFICATION: A POTENTIAL NATURAL HERBAL COAGULANT FOR WATER TREATMENT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 3, page no.d682-d691, March-2022, Available :http://www.jetir.org/papers/JETIR2203385.pdf

"AN IN-VITRO STUDY ON ANTI-BACTERIAL ACTION OF DHAVĀDI BHASMA IN WATER PURIFICATION: A POTENTIAL NATURAL HERBAL COAGULANT FOR WATER TREATMENT", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 3, page no. ppd682-d691, March-2022, Available at : http://www.jetir.org/papers/JETIR2203385.pdf