• K. Umashankar
• P. Giridhar

In the present study establishment of in vitro callus cultures of D. hamiltonii on MS medium followed by its biomass augmentation trials in Erlenmeyer conical flask and stirred tank bioreactor (STB) was investigated. The callus suspension culture grown in 150mL Erlenmeyer flask showed 9.1 g FW per 50 ml culture (182 gL-1 FW of biomass) and 0.097±0.21mg 100g-1 DW of 2H4MB in 4 weeks of growth. The methanol extract of the callus showed 13.1±0.2 mg 100g1 DW and 0.46±0.03 mg 100g-1 DW of TPC and TFC respectively. Whereas in callus culture grown in 2 lit medium in STB, 33.5 g FW of biomass was recorded and 0.024± 0.007 mg 100g1 DW 2H4MB upon 7 days growth. The methanol extract of fermenter-grown-callus showed 2.13± 0.39 mg 100g1 DW and 0.12± 0.4 mg 100g-1 DW of TPC and TFC respectively. The study infers D. hamiltonii callus culture in a bioreactor can be an alternative method for producing vanillin isomer in large-scale production and further optimization of culture conditions in this regard is required.

Callus suspension cultures, 2H4MB, Stirred tank bioreactor, Swallow root

"Biomass production and bioactive secondary metabolites of bioreactor grown callus suspension cultures of Swallow root (Decalepis hamiltonii Wight & Arn.)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 3, page no.g153-g158, March-2022, Available :http://www.jetir.org/papers/JETIR2203624.pdf

"Biomass production and bioactive secondary metabolites of bioreactor grown callus suspension cultures of Swallow root (Decalepis hamiltonii Wight & Arn.)", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 3, page no. ppg153-g158, March-2022, Available at : http://www.jetir.org/papers/JETIR2203624.pdf