• Dr.J.SURESH KUMAR
• Mr.V. Vetrivel

Tissue culture experiments on precursors of this herb will definitely improve to attain a greater improved and increased productivity. Hence, the present study has been undertaken the objectives were selection of explant, induction of callus, root and shoot and hardening of seedlings. The selected medicinal plant was Jatropha curcas L. Different concentration and combinations of growth regulators such as IAA, NAA, 2,4-D and BAP were used. It was observed that embryo axis explants showed better growth response like enlargement and initiation of callus. Therefore, only embryo axis explants of J. curcas L. were used throughout the present study. Embryo axis exhibited higher growth in the media supplemented with 5mg/l of 2,4-D and NAA; 2 mg/l of IAA and 1mg/l of BAP.

Jatropha curcas, growth regulators, media, proliferation.

"Tissue culture studies on embryo axis of Jatropha curcas L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 4, page no.a149-a161, April-2022, Available :http://www.jetir.org/papers/JETIR2204020.pdf

"Tissue culture studies on embryo axis of Jatropha curcas L.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 4, page no. ppa149-a161, April-2022, Available at : http://www.jetir.org/papers/JETIR2204020.pdf