• Shivam Pandey
• Dr Sourabhi Dutta
• Dr Monika Tyagi
• Dr Richa Yadav

A simple HPLC-UV method was developed and validated for the determination of Caffeine. Standard caffeine was separated through c-18 column (YMC ODS 5μm, internal diameter 4.6 nm and length 250 mm) using methanol and water (80:20) as a mobile phase. The peak response time for caffeine was observed at 7.77 minutes using UV detector set at 210 nm. Method validation parameters viz. linearity, sensitivity (LOD & LOQ), repeatability and recovery were assessed for the detection of caffeine. Linear dynamic range was 1-10μg/ml with correlation coefficient (R2) of 0.9998. The LOQ and LOD were found to be 0.5 and 1.5μg/ml respectively. Relative standard deviation for retention time for intraday repeatability was 1.9 and for inter-day 1.93%. Recovery was measured by spiking blank samples and the results of recovery were greater than 90%. The validated method was finally adapted to determinate caffeine content in respective samples.

Extraction, Caffeine, HPLC, Method validation.

"Analytical Method for the Determination of Caffeine by Using HPLC", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.a58-a61, May-2022, Available :http://www.jetir.org/papers/JETIR2205005.pdf

"Analytical Method for the Determination of Caffeine by Using HPLC", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. ppa58-a61, May-2022, Available at : http://www.jetir.org/papers/JETIR2205005.pdf