• Dr. Raksha Modi
• Shitika Barkale

This study evaluated the effect of CuSO4 & ZnSO4 on hypothalamic-pituitary-gonadal (HPG) axis of fresh water teleost male Anabas testudineus. The hypothalamus is projected ventrally as a round infundibulum, which is connected with the hypophysis (pituitary gland) in the brain by an indistinct pituitary stalk; this concept is called as hypothalamo-neurohypophysial system or hypothalamic-pituitary-gonadal (HPG) axis. For the study experiments were carried out by exposing the male Anabas testudineus to the test solutions containing various concentrations of heavy metals CuSO4 & ZnSO4 (copper & zink sulphate) and 50% mortality was recorded at 96 hours exposure period. The LC50 value calculated was 2.5 mg/l for CuSO4 & 3 mg/l for ZnSO4. On the basis of lethal doses test animals were exposed to the sub-lethal doses for a long period of 30 days then histopathologically and quantitatively observed the effect of heavy metals on neurohypophysial complex of fish. Results indicated that the neurohypophysial complex of fresh water fish Anabas testudineus is sensitive to the heavy metal pollutants and affect the spawning of fish. Effect of Copper & Zink sulphate on GSI was found statistically significant (P<0.05) after 30 days exposure between control and pollutant group.

Neurohypophysial complex, Hypothalamic-Pituitary-Gonadal (HPG) axis, Heavy Metal Pollutant (HMP), Infundibulum.

"Study of effect of Heavy Metal Pollutants on Testis of Anabas testudineus through HPG axis.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.c709-c715, May-2022, Available :http://www.jetir.org/papers/JETIR2205292.pdf

"Study of effect of Heavy Metal Pollutants on Testis of Anabas testudineus through HPG axis.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. ppc709-c715, May-2022, Available at : http://www.jetir.org/papers/JETIR2205292.pdf