Abstract
Annatto is a yellow-orange-red colorant derived from the resinous coating of the seeds of the plant Bixa orellana L., grown in Central and South America, India, and East Africa. Annatto has a long history of use and is available as oil or water solutions, suspensions, emulsions, encapsulated products, and spray-dried powders. Various parts of this tree are thus used for the treatment of conditions such as microbial infections, sunstroke, tonsilitis, burns, leprosy, pleurisy, apnoea, rectal discomfort, headache and for the treatment of small burns, poisoning, thinning hair, headaches, gonorrhea, skin diseases, nausea and vomiting, snake bites etc. This tree has also been suggested to possess antifertility, anticancer, antidiabetic, antifungal, antimicrobial, hepatoprotective, cardio protective, antiemetic, antispasmodic, analgesic, adaptogenic and diaphoretic actions. Previous phytochemical investigations have revealed the presence of several carotenoid compounds including bixin and norbixin some terpenoids, to cotrienols, and flavonoids in Bixa orellana, seeds. Among these, carotenoids are widely used in biological process such as vitamin A activity, cancer preventing agents, protective agent against cardiovascular diseases and decreasing effect of cataract and age related degeneration. Bixa seeds are used as purgative, antipruritic and for buccal tumors. In carotenoids, bixin is one of the most effective biological compounds and contribute to the cell protection and tissue damage against free radicals. The present study pretends to explore to determines and evaluate the antibacterial, phytochemical activities of Bixa orellana L.