• Sanjeeda Sayed Mohammed
• Hitesh Gurav

Antimicrobial drug resistance has been identified as one of the threat in treatment of infectious diseases. With an aim to achieve alternative classical antibiotic treatments there have been strong research focus on nanoparticles particularly silver nanoparticles. Green synthesis of silver nanoparticles is usually preferred over chemical synthesis since it environmental and eco friendly method. The present work evaluates the antibacterial, antioxidant, antibiofilm potency of biosynthesized silver nanoparticles from Catharanthus roseus leaf extract. The biosynthesized silver nanoparticles exhibit a notable potential for producing spherical and stable AgNPs which was characterized by UV- Vis Spectroscopy , Scanning electron microscopy and Fourier transformed infrared spectroscopy .The antioxidant activity of C.roseus and biosynthesized AgNPs was determined by DDPH method. Both C.roseus extract and biosynthesized AgNPs showed strong antioxidant activity .The biosynthesized silver nanoparticle was tested against the pathogenic organisms Staphylocccus.aureus, Pseudomonas aeruginosa by agar well diffusion method. The AgNPs exhibited effective antibacterial activity by creating a zone of inhibition against these micro-organisms. Biofilms formation of these micro- organisms was attained using Congo red agar method . Among these bacterial strains S.aureus exhibited strong biofilm formation by producing black coloured colonies on Congo red agar medium while P.aeruginosa being the weak biofilm producers. The biofilm eradication was evaluated by which showed 70% and 74% biofilms reduction of S.aureus and P.aeruginosa respectively. However, the efficiency of biosynthesized silver nanoparticles from C.roseus was found to have great antioxidant, antibacterial and antibiofilm effect against the P.aeruginosa, and S.aureus .

Catharanthus.roseus , Biofilm eradication , Antibacterial activity , Silver nanoparticles , antioxidant activity ,Green synthesis

"STUDY OF COMBINED EFFECT OF SILVER & CATHARANTHUS SILVER NANOPARTICLES ON CLINICAL PATHOGENS P. AERUGINOSA AND S.AUREUS CAUSING SKIN INFECTION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.f746-f759, May-2022, Available :http://www.jetir.org/papers/JETIR2205685.pdf

"STUDY OF COMBINED EFFECT OF SILVER & CATHARANTHUS SILVER NANOPARTICLES ON CLINICAL PATHOGENS P. AERUGINOSA AND S.AUREUS CAUSING SKIN INFECTION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. ppf746-f759, May-2022, Available at : http://www.jetir.org/papers/JETIR2205685.pdf