• NANCY
• UMA KUMARI

HCoV-NL63 main protease (M pro), also called 3C-like protease (3CL pro), plays a vital role in mediating viral replication and transcription by catalyzing the cleavage of replicase polyproteins (pp1a and pp1ab) into functional subunits. Human coronavirusNL63 (HCoV-NL63), which belongs to the genus alphacoronavirus, mainly infects children and the immunocompromised and is responsible for a series of clinical manifestations, including cough, fever, rhinorrhea, bronchiolitis, and croup. HCoV-NL63, which was first isolated from a seven-month-old child in2004, has led to infections worldwide and accounts for 10% of all respiratory illnesses caused by etiological agents. Moreover, M pro is highly conserved among all coronaviruses, thus making it a prominent drug target for antiviral therapy. The crystal structures of HCoV-NL63 M pro in 3D form the show at different pH values are reported at resolutions of up to 1.78 Å. Comparison with (M pro) from other human beta coronaviruses such as SARS-CoV-2 and SARS-CoV reveals command distinct structural features in different genera and extends knowledge of the diversity, function and evolution of coronaviruses.

Keywords: Structure Analysis, COBALT, Structure Validation, HCOV-NL63, Childhood Viral infections, E-Value, Phylogenetic Analysis.

"BIOINFORMATICS PIPELINE TO STUDY THE VIRAL SEQUENCE OF HUMAN CORONAVIRUS NL63 PROTEASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.i122-i129, May-2022, Available :http://www.jetir.org/papers/JETIR2205916.pdf

"BIOINFORMATICS PIPELINE TO STUDY THE VIRAL SEQUENCE OF HUMAN CORONAVIRUS NL63 PROTEASE", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. ppi122-i129, May-2022, Available at : http://www.jetir.org/papers/JETIR2205916.pdf