UGC Approved Journal no 63975(19)

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Published in:

Volume 9 Issue 5
May-2022
eISSN: 2349-5162

UGC and ISSN approved 7.95 impact factor UGC Approved Journal no 63975

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Published Paper ID:
JETIR2205A70


Registration ID:
403349

Page Number

j537-j543

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Title

PARTIAL PURIFICATION OF CELLULASE PRODUCED BY CELLULOMONAS SPP AND DETERMINATION OF ITS ENZYME ACTIVITY AND ENZYME KINETIC PARAMETERS

Abstract

Cellulose degrading bacteria was isolated and was purified. Cellulase extraction was carried out and was optimized. In this study, enzyme kinetic parameters were determined. The Km of the enzyme is 12.04 μmoles whereas Vmax is 5.88 μmoles/ml/min. The enzyme was then further partially purified by ammonium sulphate precipitation and dialysis method. In ammonium nitrate precipitation, the enzyme showed maximum precipitation at 60% of ammonium sulphate. The protein content was found to be 518 μg/ml. The enzyme purified by ammonium sulphate precipitation showed the yield of 68.91%. The specific activity was increased from 0.74 U/mg to 1.274 U/mg. The purification factor was 1.722-fold. After dialysis, the concentration was increased from 0.279 μg/ml to 0.3906 μg/ml, thus, the enzyme was purified. The bacterial isolate was then applied to some of the leading industrial biproducts like sugarcane bagasse, paper pulp and sugarcane molasses, which are the biproducts of sugarcane mills, paper industry and sugar industries respectively.

Key Words

Extraction, parameters, precipitation, dialysis, applied.

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"PARTIAL PURIFICATION OF CELLULASE PRODUCED BY CELLULOMONAS SPP AND DETERMINATION OF ITS ENZYME ACTIVITY AND ENZYME KINETIC PARAMETERS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 5, page no.j537-j543, May 2022, Available :http://www.jetir.org/papers/JETIR2205A70.pdf

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2349-5162 | Impact Factor 7.95 Calculate by Google Scholar

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 7.95 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Cite This Article

"PARTIAL PURIFICATION OF CELLULASE PRODUCED BY CELLULOMONAS SPP AND DETERMINATION OF ITS ENZYME ACTIVITY AND ENZYME KINETIC PARAMETERS", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 5, page no. ppj537-j543, May 2022, Available at : http://www.jetir.org/papers/JETIR2205A70.pdf

Publication Details

Published Paper ID: JETIR2205A70
Registration ID: 403349
Published In: Volume 9 | Issue 5 | Year May-2022
DOI (Digital Object Identifier):
Page No: j537-j543
Country: Khamgoan, Maharashtra, India .
Area: Biological Science
ISSN Number: 2349-5162
Publisher: IJ Publication


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