• Dr.S.P.Kamala Nalini
• Dr.K.Vijayaraghavan

Abstract - In this present investigation the effect of sulphuric acid pretreatment, steam explosion and its combined effect were evaluated in biohydrogen generation during the anaerobic fermentation on Chara the macro algal species. The results showed that acid pretreatment followed by steam explosion was proved to be the best option among the pretreatment methods studied. The optimum sulphuric acid concentration was found to be at 2% for a reaction period of 90 min at 80°C, whereas the steam explosion exhibited an optimum value of 20 min at 121°C and 15 psi during pretreatment process. Clostridium species was isolated by heat treatment technique from the agriculture soil growing tomatoes. The isolated Clostridium species was effective in hydrogen production using Chara species as the substrate. Irrespective of pretreatment option, the predominant volatile fatty acids were found to be acetate > propionate > butyrate. Anaerobic digestion of acid pretreated biomass coupled with steam explosion resulted in a acetate to propionate ratio of 4.3, while acetate to butyrate ratio was 6.7.

Algal biomass; anaerobic digestion; Chara sp.; Clostridium sp.; biogas; methane; wastewater

"Effect of anaerobic digestion on algal biomass Chara Species in Biohydrogen generation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.a224-a236, June-2022, Available :http://www.jetir.org/papers/JETIR2206025.pdf

"Effect of anaerobic digestion on algal biomass Chara Species in Biohydrogen generation", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppa224-a236, June-2022, Available at : http://www.jetir.org/papers/JETIR2206025.pdf