• Fathima sumaya
• Dr. rohini .A
• Dr. moossa p.p
• A. lakshman
• V. priyanka

One of the main methods for plant anatomy study is the analysis of thin, transparent, and stained tissue sections. Synthetic dyes traditionally used in anatomical studies might be expensive and produced by specific companies. In contrast, the use of alternative dyes can both represent an inexpensive substitute as well as an environmentally friendly option for conducting plant anatomy studies. In this study, powder of Artocarpus heterophyllus tree bark were used, Transversal sections of plants obtained using the freehand cutting technique were stained using ethanol solution and this powder mixed with 85% ethanol solution showed higher efficiency in tissue contrast, allowing greater solubility of dye powder and better solution interaction with the plant tissues. Quantitative phytochemical analysis of this species exhibited the presence of glycoside, phytosterol, phenol, flavonoid. The results pertaining to GC-MS analysis lead to the identification of a number of compounds from gas chromatography fractions of the ethanolic extract of Artocarpus heterophyllus .The results revealed that the presence of different phytoconstituents .This findings suggest that the developed method can be useful in mixed practical classes of plant anatomy, chemistry, and/or biochemistry, both at high school as well as undergraduate levels.

Artocarpus heterophyllus, Freehand cutting, GC-MS

"Utilisation of dye isolated from Artocarpus heterophyllus tree bark for botanical microtechnique", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.a466-a471, June-2022, Available :http://www.jetir.org/papers/JETIR2206052.pdf

"Utilisation of dye isolated from Artocarpus heterophyllus tree bark for botanical microtechnique", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppa466-a471, June-2022, Available at : http://www.jetir.org/papers/JETIR2206052.pdf