• Dr.R.Mahenthiran
• Sashtika A C
• Reshma S
• Reenesh Amjith R
• Sivanandham K, Sonali Pathak P

Milk being rich in the protein casein is a significant food of nutrition for immense population on earth. It is the primary source of nutrition for young mammals and human infants. Some important nutrients like calcium, phosphorous, vitamin-B, potassium, vitamin-D and proteins especially casein. Milk supplement in daily diet makes the bones stronger and prevent them from fractures and other skeletal diseases. As milk is highly nutritious, it serves as a substrate for the growth of many micro-organisms especially bacteria like Escherichia coli, Mycobacterium bovis, Salmonella sps, Listeria monocytogenes, Yersienia enterocolifica, Camphylobacter jejuni, etc. the presence of these microbes in milk reduces the quality of milk and when consumed causes various diseases to the consumer. So it is highly necessary to ensure the quality of milk before consumption. Methylene Blue Reduction Test is usually performed for the qualitative analysis of milk. When the bacterial load in milk increases, methylene blue (indicator) gets reduced due to the decrease of oxidation-reduction potential of milk from 0.06V-0.01V. The organisms present in the milk samples were isolated, identified and characterized using staining techniques like Gram’s staining and lacto phenol cotton blue staining and various biochemical tests like catalase test, oxidase test, coagulase test, urease test, citrate utilization test, MR-VP test, motility test and carbohydrate fermentation test were also performed. Staphylococcus sps, Streptococcus sps, Klebsiella sps and Lactobacillus sps were tested for sensitivity reactions against various antibiotics like erythromycin, glindamycin, gentamycin, ciprofloxacin, amoxicillin, penicillin, cephalosporin and streptomycin.

Milk quality, MBRT, milk spoiling microbes, milk spoilage.

"Qualitative analysis of milk samples and isolation, identification and characterization of micro-organisms present in the milk samples", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.b441-b454, June-2022, Available :http://www.jetir.org/papers/JETIR2206163.pdf

"Qualitative analysis of milk samples and isolation, identification and characterization of micro-organisms present in the milk samples", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppb441-b454, June-2022, Available at : http://www.jetir.org/papers/JETIR2206163.pdf