• PRADIP KUMAR BANDYOPADHYAY

The objective of this study aimed to evaluate the effect of mitotic poisoning in Jatropha curcas L. by colchicine induction in two experiments. In Experiment 1, inflorescences of physic nut were treated under different colchicine concentrations by dropping the solution directly at the stage that the frequency of male flower at microspore stage. In Experiment 2, seeds were soaked in different concentrations of colchicine at various durations. In Experiment 1 and 2, the results showed that the increasing of colchicine concentration likely to decreased size of guard cells, plant height and stomatal density. However, significant different among those parameters was not observed. The ploidy level determined from leaf cells of plants by flow cytometry in both experiments showed almost of plants were disomic (2x). However, in experiment 1, incomplete aneuploidy or mixoploid plants that mixing between trisomic (2x + 1) (at 0 mM colchicine) and tetrasomic (2x + 2) (at 2.5 mM colchicine) with disomic were also found. In experiment 2, incomplete aneuploid plants were composed of trisomic, tetrasomic and pentasomic (2x + 3) with disomic. The mixoploid plant of disomic, trisomic and hexasomic (2x + 4) was also detected when concentrations of colchicine and times for soaking duration were increased.

Mitotic Poisoning, Jatropha curcas L., Colchicine , Mixoploidy; Aneuploids

" Experiments on Effect of Mitotic Poisoning by Colchicine in Jatropha curcas L. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.c227-c236, June-2022, Available :http://www.jetir.org/papers/JETIR2206230.pdf

" Experiments on Effect of Mitotic Poisoning by Colchicine in Jatropha curcas L. ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppc227-c236, June-2022, Available at : http://www.jetir.org/papers/JETIR2206230.pdf