• Dr. Reena G. Desai
• Riya V. Lad
• Khushi P. Shah

This study has been undertaken to investigate the determination of Laccase and its application in bioremediation. Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas. Laccase producing bacteria were isolated from local regions of Valsad district using an enrichment culture technique. The bacterial isolates were grown on Luria Bertani agar medium containing 0.5 mM guaiacol for detection of extracellular laccase enzyme. The bacterial isolates produced reddish brown coloration around the colonies was further purified and plate assayed on the same media for laccase enzyme production. In liquid culture, the highest laccase production (0.048 U/ml) was achieved on the 24-48 h of incubation at room temperature and pH 7.0. The Luria Bertani broth medium was optimized with different carbon sources as well as nitrogen sources and gives maximum laccase activity at carbon source glucose (16.66 U/ml) and nitrogen source fallen leaves (33.20 U/ml). Dye decolorization were done with Reactive blue 171 at different concentrations 100ppm, 200ppm and 300ppm and dye were decolorized 86.61%, 78.08% and 76.30% respectively. Bioremediation of pesticides like Cypermethrin and Chlorpyriphos was done which was 71.73% and 56.73% respectively.

Isolate, sources, laccase, Guaiacol, Bioremediation, Pesticide

"Isolation of Laccase producing microorganisms and production of extracellular laccase enzyme and its potential application in decolorization and degradation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.c776-c782, June-2022, Available :http://www.jetir.org/papers/JETIR2206295.pdf

"Isolation of Laccase producing microorganisms and production of extracellular laccase enzyme and its potential application in decolorization and degradation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppc776-c782, June-2022, Available at : http://www.jetir.org/papers/JETIR2206295.pdf