• Shital Popat Gaikwad
• Dr. Monika Gopal Shinde
• Dr. Manojkumar Sheshroa Patil
• Miss. Priyanka Maruti Adat
• Miss. Pranali Vikas Dhasade

A reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of Ipratropium Bromide and Levosalbutamol in combined pharmaceutical formulation is developed. A Chromatographic separation carried out at 30oc temperature on Agilent Zorbax SB-Aq (250 x 4.6 mm, 5 µ) column. Mobile phase consist of Acetonitrile: 0.1 % Perchloric acid (50:50, % v/v) with flow rate 0.8ml/min and the injection volume was 10 μl. The detection was done by Uv detector at 220nm and the run time was kept 7 min. Acetonitrile: Water (50:50, % v/v) used as a diluent. The retention time for Ipratropium Bromide and Levosalbutamol was at 4.14 and 3.03 respectively. The linearity range for Ipratropium Bromide and Levosalbutamol is 12-18 µg/ml and 36-54 µg/ml. The %RSD for accuracy was found to be less than 2%. High recovery of Ipratropium Bromide (98.8-100.1%) and Levosalbutamol (99.8-101.7%) indicates accuracy of the method. Validation parameters such as accuracy, linearity, precision, system suitability are considered as per International Conference on Harmonization guidelines. Based on all above parameters the proposed method was found to be simple, accurate, precise and reproducible and can be used for simultaneous estimation of Ipratropium Bromide and Levosalbutamol.

Ipratropium Bromide, Levosalbutamol, RP-HPLC

"Analytical Method Development and Validation of Rp-hplc Method for Simultaneous Estimation of Ipratropium Bromide and Levosalbutamol in Combined Dosage Formulation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.h280-h291, June-2022, Available :http://www.jetir.org/papers/JETIR2206733.pdf

"Analytical Method Development and Validation of Rp-hplc Method for Simultaneous Estimation of Ipratropium Bromide and Levosalbutamol in Combined Dosage Formulation.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. pph280-h291, June-2022, Available at : http://www.jetir.org/papers/JETIR2206733.pdf