• Manisha Jagtap
• Lahu Hingane
• Ashwini Aswar
• Pritam Dhonde

Analytical method validation according to USP is performed to ensure that an analytical methodology is accurate, specific, reproducible, precise and rugged over the specified range that an analyte will be analysed. High Performance Liquid Chromatography is also called as High-Pressure Liquid Chromatography. It is very popular analytical technique. High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture. Reversed phase HPLC (RP-HPLC) has a non-polar (hydrophobic)stationary phase and an aqueous, moderately polar mobile phase. e.g. blends of water and methanol or acetonitrile. It deals with the rule of hydrophobic collaborations thus the more nonpolar the material is, the more it will be held. Isavuconazole is an odorless, white crystalline powder soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), it is sparingly soluble in aqueous buffers

RP-HPLC, HPLC, Isavuconazole, reverse phase, analytical method

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF ISAVUCONAZOLE IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.i357-i373, June-2022, Available :http://www.jetir.org/papers/JETIR2206845.pdf

"DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF ISAVUCONAZOLE IN PHARMACEUTICAL DOSAGE FORM", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppi357-i373, June-2022, Available at : http://www.jetir.org/papers/JETIR2206845.pdf