• Sowmya K L
• Dr. Ramalingappa B

CRISPR is an indispensable tool in biological research. Recent advances in genome engineering technologies based on the CRISPR- associated RNA- guided endonuclease Cas 9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, and agricultural. In this review, we describe the development and applications of Cas9 for a variety of research or translational applications, highlighting the potential of this technology for cancer treatment as well as future and prospective of CRISPR.

CRISPR-Cas system , CAS gene nomenclature. Type I,II,III and IV.

"CRISPR/CAS TECHNOLOGY: PRINCIPLE AND CLASSIFICATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 6, page no.j352-j360, June-2022, Available :http://www.jetir.org/papers/JETIR2206948.pdf

"CRISPR/CAS TECHNOLOGY: PRINCIPLE AND CLASSIFICATION", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 6, page no. ppj352-j360, June-2022, Available at : http://www.jetir.org/papers/JETIR2206948.pdf