• Jyoti Kulkarni

Fenugreek (Trigonella foenum-graecum) is an ancient and very well-known medicinal plant used in the preparation of traditional medicine in various parts of the world. India is one of the biggest cultivators of Fenugreek. Fenugreek is famous for its medicinal properties, the protein source in diet, Rhizobium associated symbiotic nitrogen fixation in root nodules. The present study focused on the isolation and characterization of a Rhizobium strain from fenugreek root nodules and its application as a biofertilizer. The characterized Rhizobium isolates are gram-negative, rod-shaped, mucous, and acid-producing. The isolates are found to be pH and temperature sensitive. As a sole carbon source, it usually utilizes sucrose, glucose, and starch. The Rhizobium species which are isolated from fenugreek roots are having the ability to synthesize industrially important enzymes; cellulose and amylase. Amylase is among the most important enzymes and is of great significance in present-day biotechnology..

Fenugreek, Rhizobium, Biofertilizers, Enzyme production, Root nodules.

" ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM ROOTS OF FENUGREEK AND ITS USE AS A BIOFERTILIZER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 7, page no.e272-e277, July-2022, Available :http://www.jetir.org/papers/JETIR2207434.pdf

" ISOLATION AND CHARACTERIZATION OF RHIZOBIUM FROM ROOTS OF FENUGREEK AND ITS USE AS A BIOFERTILIZER", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 7, page no. ppe272-e277, July-2022, Available at : http://www.jetir.org/papers/JETIR2207434.pdf