• Rutuja S. Gavali
• Mr.N.K.Nogal

In the present work, formulate and evaluate ocimum sanctum loaded microsponge based gel for anti fungal activity. Microsponge varied with drug, polymer ratios were prepared by quasi emulsion solvent diffusion method. In this, ethyl cellulose used as a polymer with plasticizer triethyl citrate and ethyl acetate used as a solvent. Physico chemical properties of ocimum sanctum show presence of alkaloids, carbohydrate, terpenoids, flavonoids, protein in the extract. The absorbance maxima of ocimum sanctum were found to be 284nm. FTIR study of physical mixture (i.e. drug, polymer, PVA) showed all the characteristic peaks of drug and polymer and it revealed the major functional group of drug hence there is no interaction between drug and polymer. Prepared microsponges were studied for particle size (µm), entrapment efficiency (%), physical characterization, scanning electron microscopy (SEM), differential scanning calorimtery (DSC). Particle size is within range 5 µm -300 µm and SEM revealed the result that microsponges are porous and spherical in shape. Entrapment efficiency of optimized batch (F5) was found to be 69.12%. The optimized formulation of microsponges was then incorporated into gel by using carbapol934 as a polymer. The gel is evaluated as physical characterization, pH, spredability, viscosity, in vitro antifungal activity. Characterization of gel shows better results as pH in range 5.2-5.4, spredability is in range of 7.2 gcm/sec and viscosity was found to be 39890 cps. Minimum inhibitory concentration(MIC) of ocimum sanctum shows antifungal activity in the concentration 2mg/ml .The comparative antifungal study was performed in ocimum sanctum plain gel and ocimum sanctum microsponge loaded gel by agar well diffusion method by using candida albicans species. It shows that ocimum sanctum loaded microsponge based gel having better zone of inhibition than ocimum sanctum plain gel. Hence, encapsulation of ocimum sanctum in microsponge based gel resulted in efficacious carrier system in terms of stability as well as safety of this herbal extract along with handling benefits.

Ocimum sanctum, micro sponges, C. albicans, gel, anti fungal

"Formulation And Evaluation Of Ocimum Sanctum Loaded Microsponge Based Gel For Topical Delivery.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 7, page no.e532-e541, July-2022, Available :http://www.jetir.org/papers/JETIR2207467.pdf

"Formulation And Evaluation Of Ocimum Sanctum Loaded Microsponge Based Gel For Topical Delivery.", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 7, page no. ppe532-e541, July-2022, Available at : http://www.jetir.org/papers/JETIR2207467.pdf