• Dr. Raghu Nataraj
• Mr. Nitin K R
• Dr. Bindu J

Psychological stress is the body’s response for stressors. Fear, anxiety and depression are widely attributed as a potent causative stress factors to instill PCOS in non-obese women with oligo/anovulation being the symptom. Recent reports have shown more than 90% of the patients are under moderate to high psychological stress. The study aims to examine the effect of randomized chronic stress on the variation of estrous cycle in rats to deepen our understanding of the effect of psychological stress in women leading to oligo/anovulation and hence manifesting into PCOS. A total of 20 Rattus norvegicus strains were grouped as control and stress groups with 10 animals each. Stress group of animals were exposed to different parameters of stressors for a study of 30 days with control group of animals being kept undisturbed for the same number of days. Vaginal fluid from all the grouped animals was collected, smeared on a clean glass slide, stained and observed under a microscope. At the end of the study, animals were euthanized and the ovarian morphology was examined against the observed variation in estrous cycle via vaginal smear analysis to the cystic ovaries. A significant difference with a value of p=0.05 for the estrous cycle, i.e., 4.3±0.46 estrous cycles in control group animals and 2.5±0.5 estrous cycles in stress group of animals during the study period were observed. Further a significant difference with p=0.001 for number of days to complete one estrous cycle, i.e., 5.8±0.98 days/estrous cycle in control and 8.5±1.28 days/estrous cycle in stress group animals were noted and the morphological change in the ovaries of stress group animals to the control group of animals was further correlated and the result established. Variations were significantly observed across different phases of estrous cycle correlating with the effect of chronic stress induction. The study identifies the effect of randomised chronic stress induction in altering the estrous cell cycle of rat, effect in the formation of cyst in the animal ovaries and hence establishing the possible link between chronic stress exposure and PCOS induction in vivo.

"A STUDY ON THE EFFECT OF RANDOMIZED CHRONIC STRESS ON ESTROUS CYCLE AND OVARIAN CYST IN RATTUS NORVEGICUS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org), ISSN:2349-5162, Vol.9, Issue 8, page no.c860-c867, August-2022, Available :http://www.jetir.org/papers/JETIR2208291.pdf

"A STUDY ON THE EFFECT OF RANDOMIZED CHRONIC STRESS ON ESTROUS CYCLE AND OVARIAN CYST IN RATTUS NORVEGICUS ", International Journal of Emerging Technologies and Innovative Research (www.jetir.org | UGC and issn Approved), ISSN:2349-5162, Vol.9, Issue 8, page no. ppc860-c867, August-2022, Available at : http://www.jetir.org/papers/JETIR2208291.pdf